For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)
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So far, the long-term change of weight after discontinuation of AOMs is still lacking of systematic evaluation
doi.org/10.1056/NEJMoa2307563 10Jastreboff A.M
