Tobacco BY-2 cells (RIKEN BRC through the National BioResource Project) were cultured in modified Linsmaier and Skoog medium (pH 5.5), which contained Murashige and Skoog Plant Salt Mixture (Wako), 30 g l 1 sucrose, 0.2 g l 1 KH 2 PO 4 , 100 mg l 1 myo-inositol, 1 mg l 1 thiamine-HCl, 0.2 mg l 1 2,4-dichlorophenoxyacetic acid and NaOH, in the dark at 25 C with agitation at 130 rpm
A diet high in processed foods, fats, and refined sugars can increase oxidative stress within the body
Mishra T, Sasanka KK, Sudha Ty S, Kumar D, Ps S, Bano G, et al
In infants, breast milk and infant formula are the main sources of Mn (Stastny et al., 1984