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Allow vials to reach room temperature for 15 to 30 minutes before opening to prevent condensation Disinfect rubber stoppers with alcohol swab and allow complete evaporation Draw the calculated volume of bacteriostatic water into syringe Angle needle against vial wall and slowly inject water down the side, allowing it to gently slide onto powder Never squirt water directly onto lyophilized powder as this damages peptide structure Gently swirl or roll vial between palms to dissolve

Cardiac injury: Myocardial IR during cardiac surgery generates substantial mtDAMPs release, contributing to post-cardiotomy syndrome (fever, leukocytosis, organ dysfunction) and low cardiac output syndrome