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In vivo , on pregnant day 2, prior to blastocysts entering the uterine cavity, 25 l of the siRNA/RNAi-Mate mixture (the final concentration of GLUT4-siRNA was 500 M) was directly injected into one side of the mouse uterine horns and, with the other side injected with the same amount of non-specific siRNA as the control, followed by comparison of the changes of glucose concentration in the uterine fluid, the expressions of key uterine receptivity markers, embryonic development, and implantation on pregnant day 4 or day 5

Subsequently, animals were placed at the end of one arm and allowed to explore freely among the three arms of the Y-maze for 8 minutes

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Similarly, in rheumatoid fibroblast-like cells, in which miR-193a-3p also targets IGFBP-5, miR-193a-3p inhibition leads to IGFBP-5 induction and enhanced apoptosis [100]